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Crocker was in charge of construction. Construction crews comprised 12,000 Chinese emigrant workers by 1868, when they constituted eighty percent of the entire work force.  They laid the first rails in 1863. The “Golden spike”, connecting the western railroad to the Union Pacific Railroad at Promontory, Utah, was hammered on May 10, 1869. Coast to coast train travel in eight days became possible, replacing months long sea voyages and lengthy, hazardous travel by wagon trains.
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The orchestration of this dynamic instability is related to GTP binding and hydrolysis at the exchangeable or E site of tubulin. GTP binds to both and tubulin, but in the case of tubulin, GTP is found at the nonexchangeable or N site (Figure 2). Microtubule assembly requires tubulin to be charged with GTP, which is hydrolysed upon addition of the tubulin dimer to the elongating microtubule.
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