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Crocker was in charge of construction. Construction crews comprised 12,000 Chinese emigrant workers by 1868, when they constituted eighty percent of the entire work force.[5] [6] They laid the first rails in 1863. The “Golden spike”, connecting the western railroad to the Union Pacific Railroad at Promontory, Utah, was hammered on May 10, 1869.[7] Coast to coast train travel in eight days became possible, replacing months long sea voyages and lengthy, hazardous travel by wagon trains.

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The orchestration of this dynamic instability is related to GTP binding and hydrolysis at the exchangeable or E site of tubulin. GTP binds to both and tubulin, but in the case of tubulin, GTP is found at the nonexchangeable or N site (Figure 2). Microtubule assembly requires tubulin to be charged with GTP, which is hydrolysed upon addition of the tubulin dimer to the elongating microtubule.

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